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In order to confirm the spectral data of hemolytic end products, one of the fluorescent metabolite, NADH, was measured by the conventional biochemical analysis using ELISA [(HT Bio-Tech USA) with the ab65348 kit obtained from ABcam UK

Spirometry measuring forced expiratory volume in 1 s (FEV1) and the FEV1/forced vital capacity (FVC) ratio helps quantify airflow limitation indicative of obstructive lung diseases

This approach provided detectable levels of 82 VOCs and SVOCs from a single 40 mL puff and can be easily implemented by labs that do not have access to a smoking machine